Tesi etd-11272019-113007 |
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Tipo di tesi
Tesi di laurea magistrale
Autore
LO GERFO, GIULIA
URN
etd-11272019-113007
Titolo
Photochromic fluorescent proteins for high-resolution cellular imaging
Dipartimento
CHIMICA E CHIMICA INDUSTRIALE
Corso di studi
CHIMICA
Relatori
relatore Dott. Bizzarri, Ranieri
Parole chiave
- fluorescent proteins
- photoswitchable fluorescent proteins
- super-resolution fluorescence microscopy
Data inizio appello
12/12/2019
Consultabilità
Non consultabile
Data di rilascio
12/12/2089
Riassunto
The major goal of this thesis is the investigation of the photophysical and photochemical properties of two new mutant fluorescent proteins from the Aequorea jellyfish family, namely wQL and EYQ1L.
Our target was actually to assess whether the Q69L mutation could positively cooperate with E222Q to improve the photoswitching properties as compared to E222Q alone. In this context, we always compared the mutation effect on each couple, the green one (wQ/wQL) and the yellow one (EYQ1/EYQ1L).
All our experiments highlight the peculiar properties of wQL as RSFP, namely high bright/dark contrast ratio, high off-switching quantum yield and strong resistance to photobleaching.
The high performances of the mutant wQL in terms of photoswitching opens the way to several application in super-resolution fluroescence microscopy with possibly strong implications in terms of biological results.
Our target was actually to assess whether the Q69L mutation could positively cooperate with E222Q to improve the photoswitching properties as compared to E222Q alone. In this context, we always compared the mutation effect on each couple, the green one (wQ/wQL) and the yellow one (EYQ1/EYQ1L).
All our experiments highlight the peculiar properties of wQL as RSFP, namely high bright/dark contrast ratio, high off-switching quantum yield and strong resistance to photobleaching.
The high performances of the mutant wQL in terms of photoswitching opens the way to several application in super-resolution fluroescence microscopy with possibly strong implications in terms of biological results.
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