Tipo di tesi
Tesi di specializzazione (5 anni)
Titolo
Preclinical investigation of Plasminogen as inhalable therapy for ARDS
Dipartimento
PATOLOGIA CHIRURGICA, MEDICA, MOLECOLARE E DELL'AREA CRITICA
Corso di studi
ANESTESIA, RIANIMAZIONE, TERAPIA INTENSIVA E DEL DOLORE
Riassunto (Italiano)
The aim of our study is to investigate the effect of PLG in preclinical studies both in vitro and in vivo.
We studied the profile of deposition of PLG depending on different respiratory flow curves, as in-vitro models.
Using a breathing simulator (BRS) and a next generation impactor (NGI), we studied the profile of deposition of the drug simulating the normal and the injured respiratory system.
The demonstration of how the drug spreads into the respiratory system, and how it changes, depending on different models of breathing, could be useful in the practical clinic for the application of this therapy in patients suffering from ARDS.
Moreover, we studied the direct effect of PLG on sane and ARDS induced mice lungs.
The in-vivo study evaluated the effect of PLG on ARDS in short (1 day) and long term (14 days) to evaluate its potential on different pathogenetic pathways of inflammatory response.
