Tipo di tesi
Tesi di laurea magistrale
Titolo
In vivo selection of intrabodies for post-translational protein targeting
Corso di studi
BIOLOGIA MOLECOLARE E CELLULARE
Parole chiave
- antibody
- bait
- intrabodies
- post-translational modification
- protein silencing
- protein targeting
- PTM
- scFv
- single domain antibodies
- yeast two-hybrid
Data inizio appello
18/10/2012
Consultabilità
Non consultabile
Data di rilascio
18/10/2052
Riassunto (Italiano)
Intracellular Antibodies (Intrabodies) are engineered antibodies (or antibody domains) ectopically expressed in several cell types to specifically perturb the function of intracellular antigens. (Biocca & Cattaneo, 1995)
Since Antibodies are proteins whose folding depends on the formation of critical disulphide bonds, the successful recognition of the physiological target, located in a reducing environment such as the cytoplasm, is somewhat unpredictable.
For this reason, a general and reliable method for the intracellular selection of Intrabodies was developed (Visintin et al, 1999). Based on the two-hybrid assay in yeast, the so-called “IACT” (Intracellular Antibody Capture Technology) allows us to isolate specific intrabodies, from diverse cDNA libraries of antibody domains [either ScFv (Single Chain Fragment Variable domains) or dAbs (single domain antibodies)] directly in the cytosolic environment, avoiding any protein manipulation and solving the folding problem.
