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Tesi etd-09172022-152304


Tipo di tesi
Tesi di laurea magistrale
Autore
MALVESTIO, COSIMO
URN
etd-09172022-152304
Titolo
A new triethyloxonium tetrafluoroborate (TEOT) based derivatization method for the quantitative analysis of Methionine and SelenoMethionine by gas chromatography - mass spectrometry (GC-MS)
Dipartimento
CHIMICA E CHIMICA INDUSTRIALE
Corso di studi
CHIMICA
Relatori
relatore Dott.ssa Campanella, Beatrice
Parole chiave
  • acid digestion
  • aminoacids
  • derivatization
  • gcms
  • isotope dilution
  • method development
  • metrology
  • selenized yeast
  • triethyloxonium tetrafluoroborate
Data inizio appello
17/10/2022
Consultabilità
Non consultabile
Data di rilascio
17/10/2092
Riassunto
The assessment of the aminoacidic content of food samples is of crucial importance, both from a health and an industrial point of view. Particular attention is reserved for sulfur-containing amino acids, like Methionine and Cysteine, or the main source of selenium dietary intake, Selenomethionine.
A novel method for the Gas Chromatography – Mass Spectrometry (GC-MS) determination of Methionine and Selenomethionine in food and biological samples is therefore herein proposed. A single step derivatization in aqueous medium employing triethyl oxonium tetrafluoroborate salts allows the extraction and the volatilization of the analytes as ethyl derivatives, enabling their detection in ultra-trace concentrations (low part per billions).
The method is validated by analysis of the selenized yeast Certified Reference Material SELM-1 extract, obtained by microwave assisted acid digestion with methanesulfonic acid 4M.
The main goal of this work is to offer a clean (reagents- and by-products-wise) and simple derivatization procedure as a valid alternative to the standard alkyl chloroformate methods.
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