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Tesi etd-09012020-101805


Tipo di tesi
Tesi di laurea magistrale
Autore
ZOPPI, FILIPPO
URN
etd-09012020-101805
Titolo
Hurdles and perspectives during the generation of subtype-specific neurons from postnatal astroglia of the mouse cerebral cortex.
Dipartimento
BIOLOGIA
Corso di studi
BIOLOGIA MOLECOLARE E CELLULARE
Relatori
relatore Prof.ssa Raffa, Vittoria
relatore Prof. Berninger, Benedikt
Parole chiave
  • astrocita
  • astrocyte
  • astroglia
  • caderina
  • cadherin
  • direct reprogramming
  • ECM
  • extra cellular matrix
  • fibronectin
  • fibronectina
  • IKVAV
  • matrice extracellulare RGD
  • neuron
  • neurone
  • nuclear shrinkage
  • reprogramming
Data inizio appello
21/09/2020
Consultabilità
Non consultabile
Data di rilascio
21/09/2090
Riassunto
Direct lineage reprogramming allows the conversion of one cell into another without passing through an intermediate multipotent state. Nowadays, with the overexpression of several key fate determinants is possible to generate specific neuron subtypes from astroglial cells. However, several aspects of the mechanism underlying this transdifferentiation remain unclear and the efficiency of in vitro reprogramming quite low. Here we try to improve the viability of reprogrammed cells by testing different adhesive molecules (ECM and cell junction components and mimetic compounds). Moreover, the astrocytes that undergo reprogramming show, in the first 7 days post viral infection (d.p.i.), a nuclear shrinkage reflected in a reduction of the nuclear projected area and an increase of nuclear staining intensity. Our tests using inhibitors of the acto-myosin contraction, fundamental for the tensions underlying the nuclear shrinkage, suggest that the nuclear size reduction is escorted by a cell body area contraction and is fundamental for the reprogramming itself.
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