• bEnd.3
• crosslinked
• FLIM
• galactosylcerebrosidase
• GALC
• Krabbe
• micelles
• MO3.13
• PEG
• PLGA
• polymeric
• reverse

Preliminary results of In Vitro cytocompatibility of Stabilized Reversed Micelles for the treatment of MO3.13 WT, MO3.13 GALC knock out and bEnd.3. GALC activity was measured in MO3.13 KO with HMU assay post 24- and 48- h from treatment with GALC-loaded RMs-Ang2.Two-photon excitation microscopy allowed to reveal the intrinsic fluorescence of both cells and RMs polymeric formulation, making possible the analyses based on the lifetime of the two species. Finally I made use of the FLIM technique for visualization of label-free RMs inside the mentioned cell lines, 4 h and 24 h post treatment. These preliminary results represent a starting point for the application of these nanocarriers in the delivery of hydrophilic enzymes associated with LSDs such as Krabbe disease.