• additive manufacturing
• bioinchiostri
• bioinks
• biomateriali
• biomaterials
• bioprinting
• biostampa
• carbossimetil chitosano
• carboxymethyl chitosan
• chitosano
• crosslinking
• gelatin methacryloyl
• gelatin norbornene
• gelatina
• gelatina metacriloile
• gelMA
• ionotropic gelation
• manifattura additiva
• microfluidic printing
• microfluidica
• scaffold
• tissue engineering
• UV

Bioprinting has emerged as a transformative technology in tissue engineering, offering unparalleled capabilities in fabricating complex tissue constructs. However, the limited range of suitable materials remains a significant challenge. This study aims to develop new bioinks from natural-origin materials, specifically carboxymethyl chitosan (CMCS), gelatin methacryloyl (GelMA), and gelatin norbornene (GelNB). CMCS undergoes ionotropic gelation in the presence of high ion concentrations, while GelMA and GelNB can be photocrosslinked upon exposure to UV light. The primary objective of developing these bioinks is to facilitate the construction of in vitro tissue and organ models that closely mimic native tissues. Bioprinting's ability to reproducibly and automatically produce complex 3D tissues holds promise for replicating the spatial and chemical intricacies of native tissues, advancing the field of tissue engineering. Two bioprinting techniques were employed: extrusion-based and microfluidic bioprinting. Extrusion bioprinting is the most widely used platform but faces challenges with resolution and printing speed. In contrast, integrating microfluidic devices addresses these limitations, enhancing precision and complexity in tissue constructs. Combining these approaches with novel bioinks can significantly advance the field of bioprinting, paving the way for innovative solutions in tissue engineering and regenerative medicine.