Tipo di tesi
Tesi di laurea magistrale
Titolo
Development of 3D model of non-small cell lung carcinoma for advanced imaging analysis
Dipartimento
INGEGNERIA CIVILE E INDUSTRIALE
Corso di studi
MATERIALS AND NANOTECHNOLOGY
Riassunto (Italiano)
This experimental work focuses on the development of three-dimensional in vitro model to study lung adenocarcinoma, in particular the spatial organization of proteins of the polycomb repressive complex (PRC) family. Briefly, different types of scaffolds are prepared via conventional methods, aiming to match the elastic modulus of the lung adenocarcinoma microenvironment and to resemble the morphology of the alveoli. Confocal fluorescence microscopy is exploited to assess the variation of the spatial localization of PRC proteins inside the nuclei of the cells. Comparisons between the two dimensional and three dimensional cultures are performed and based on the effect of the three dimensional culture, the material composition and the morphology of the cultured tissue.
