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Archivio digitale delle tesi discusse presso l’Università di Pisa

Tesi etd-05242017-101402


Tipo di tesi
Tesi di laurea magistrale
Autore
DAL PINO, MARCO
URN
etd-05242017-101402
Titolo
Silicon Nanopillars for T Cell Transfection
Dipartimento
INGEGNERIA DELL'INFORMAZIONE
Corso di studi
INGEGNERIA BIOMEDICA
Relatori
relatore Prof. Barillaro, Giuseppe
correlatore Prof. Voelcker, Nicolas
tutor Ing. Cozzi, Chiara
controrelatore Prof. Vozzi, Giovanni
Parole chiave
  • CD4
  • cell culture
  • electrochemical etching
  • Jurkat
  • microfabrication
  • nanopillar
  • silicon
  • T cell
  • transfection
Data inizio appello
16/06/2017
Consultabilità
Non consultabile
Data di rilascio
16/06/2087
Riassunto
The aim of this thesis is to investigate the interactions of silicon nanopillar (SiNP) arrays with human immune cells in order to achieve their effective transfection. A novel microstructuring technology, namely electrochemical micromachining (ECM), allows to fabricate SiNP arrays, featuring different heights, pitches and shapes. The SiNP arrays, adequately coated with genetic material – specifically, plasmid DNA - are used as substrates for cell seeding in order to evaluate cell viability, transfection efficiency and detachmnent capability. In particular, immune T cells, namely Jurkat (immortalized human cells) and CD4 (primary human cells) have been used to obtain, through their transfection, human CAR T cells, a cell type which is nowadays considered particularly promising for its use in personalized cancer treatments.
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