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Archivio digitale delle tesi discusse presso l’Università di Pisa

Tesi etd-04032019-175238


Tipo di tesi
Tesi di laurea magistrale
Autore
MENEGHETTI, NICOLO
URN
etd-04032019-175238
Titolo
Ultrasound modulation of neural activity in leech and zebrafish
Dipartimento
INGEGNERIA DELL'INFORMAZIONE
Corso di studi
BIONICS ENGINEERING
Relatori
relatore Prof. Micera, Silvestro
correlatore Prof. Mazzoni, Alberto
tutor Dott.ssa Dedola, Francesca
Parole chiave
  • calcium imaging
  • electrophysiology
  • leech
  • LIFU
  • neuromodulation
  • ultrasound
  • wide-field
  • zebrafish
Data inizio appello
24/04/2019
Consultabilità
Non consultabile
Data di rilascio
24/04/2089
Riassunto
Ultrasound (US) has received widespread attention as an emerging technology for non-invasive targeted neuromodulation alternative to electrical based approaches. However, little is known about the fundamental mechanism(s) by which US can excite/inhibit neurons as well as the spatiotemporal pattern of ultrasound-induced central nervous system (CNS) activity. In order to address these scientific questions, I have analyzed the data of two different experiments, both using low intensity US stimulation, at two different levels of granularity. The former consists in the analysis of individual neuron response, through intracellular electrodes, of ex-vivo N cell medicinal leech (Hirudo medicinalis). The latter concerns an in vivo exploration of the spatiotemporal patterns of the CNS obtained employing wide-field optical imaging of calcium signals in transgenic larval stages of zebrafish (Danio rerio).
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