Tipo di tesi
Tesi di dottorato di ricerca
Titolo
Proteomics study to the role of the vanilloid receptors in the
parathyroid glands.
Settore scientifico disciplinare
CHIM/08 - CHIMICA FARMACEUTICA
Corso di studi
FISIOPATOLOGIA CLINICA E SCIENZE DEL FARMACO
Riassunto (Italiano)
The parathyroid glands play an overall regulatory role in the systemic calcium
(Ca2+) homeostasis. The purpose of the present study was to demonstrate the
presence of the Ca2+
channels transient receptor potential vanilloid (TRPV) 5 and
TRPV6 in human parathyroid glands. Reverse-transcriptase PCR was carried-out
to evaluate the presence of TRPV5 and TRPV6 mRNAs in sporadic parathyroid
adenomas and normal parathyroid glands. Western blot and immunocytochemical
assays were used to assess protein expression, cellular localization and time
expression in primary cultures from human parathyroid adenoma. TRPV5 and
TRPV6 transcripts were then identified both in normal and pathological tissues. A
predominant immunoreactive band was detected at 80-85 kDa for both vanilloid
channels which appeared significantly reduced after peptide:N-glycosidase F
(PNGaseF) treatment. These channels co-localized with the calcium sensing
receptor (CASR) on the membrane surface, but immunoreactivity was also
detected in the cytosol and around the nuclei. Our data showed that western
blotting recorded an increase of protein expression of both channels in adenoma
samples compared with normal glands suggesting a potential relation with the cell
calcium signaling pathway and the pathological processes of these glands
