Sistema ETD

banca dati delle tesi e dissertazioni accademiche elettroniche

 

Tesi etd-11112014-154730


Tipo di tesi
Tesi di laurea magistrale
Autore
ALOISI, CLAUDIA
URN
etd-11112014-154730
Titolo
Biotechnological production of Isd11 and study of its state of aggregation
Struttura
CHIMICA E CHIMICA INDUSTRIALE
Corso di studi
CHIMICA
Commissione
relatore Prof.ssa Pastore, Annalisa
relatore Prof. Di Bari, Lorenzo
Parole chiave
  • protein expression
  • cluster Fe-S
  • Isd11
  • cloning
Data inizio appello
17/12/2014;
Disponibilità
completa
Riassunto analitico
Isd11 is an 11 kDa protein present only in Eukarya and involved in the biogenesis of iron-sulfur cluster. In conjunction with Nfs1 it fulfils the role of cysteine desulfurase, however its precise function is still unclear. Literature is rather lacking of information about its structure as well, above all because it is well-known to be quite unstable if expressed alone.
The final aim of this thesis was to study the aggregation of Isd11; thus it was expressed in three different vectors, in order to optimize its production, and it was characterized by NMR, CD, analytical gel filtration and SEC-MALS.
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