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Tesi etd-10242017-093841


Thesis type
Tesi di laurea magistrale
Author
CATELLI, ILARIA
URN
etd-10242017-093841
Title
The role of farnesoid X receptor in the regulation of fibroblast growth factor 15 expression by soluble fibres
Struttura
FARMACIA
Corso di studi
SCIENZE DELLA NUTRIZIONE UMANA
Commissione
relatore Dott. Fornai, Matteo
relatore Prof.ssa Testai, Lara
Parole chiave
  • Enterohepatic circulation
  • cholesterol
  • Intestine
  • Liver
  • Fibres
  • FXR
  • Bile acids
Data inizio appello
08/11/2017;
Consultabilità
parziale
Data di rilascio
08/11/2020
Riassunto analitico
Bile acids are not only simple molecules that permit digestion of fat and vitamins, but they are involved in the enterohepatic circulation to modulate their own synthesis and cholesterol turn over. Farnesoid X receptors in the ileum bound bile acids and let fibroblast growth factor 15 (Fgf15) reach the liver to down regulated cyp7A1, the rate limiting enzyme in the bile acids production from the cholesterol. We want to investigate if the different expression of Fgf15 by two diets is FXR dependent: chow diet with high fibres content and a low fibres diet. Three molecules have been used to modulate FXR expression: guggulsterone, a FXR antagonist; fexaramine, a FXR agonist; cholestyramine, a bile acids chelator. We analysed gene involved by qPCR, caspase 3 activity, hystology of ileum and liver, bile acids, triglycerides and cholesterol measurement.<br>High fibres intake is associated with a reduction in bile acid pool, associated with a decreased of lipid storage in hepatic tissue. Differential Fgf15 expression by soluble fibres seems to be independent from FXR.<br>
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