logo SBA

ETD

Archivio digitale delle tesi discusse presso l’Università di Pisa

Tesi etd-09092010-132917


Tipo di tesi
Tesi di laurea specialistica
Autore
MANNELLA, LORENZO
Indirizzo email
mannellalorenzo@gmail.com
URN
etd-09092010-132917
Titolo
Laccase activity in Trichoderma virens and comparison with the white-rot fungus Coprinus cinereus
Dipartimento
AGRARIA
Corso di studi
BIOTECNOLOGIE VEGETALI E MICROBICHE
Relatori
relatore Prof.ssa Lübeck, Mette
correlatore Dott. Guglielminetti, Lorenzo
relatore Prof.ssa Vergara, Mariarosaria
Parole chiave
  • ABTS
  • Coprinus cinereus
  • laccase
  • lignin
  • moonlight enzyme
  • textile dyes
  • Trichoderma virens
Data inizio appello
04/10/2010
Consultabilità
Completa
Riassunto
The goal of this work was to investigate laccase functions in Trichoderma virens. The strain under study, I10, was known to efficiently antagonize plant pathogens and to be involved in mycoparasitism against sclerotial fungi. For this a laccase activity could be needed to penetrate cell walls of sclerotia rind, the external layer mostly rich in melanin. Six putative laccase genes, likely associated to different biological roles, were previously identified in the T. virens genome. These possible laccase functions could be both interesting for biocontrol and biotechnological applications related to ligninolysis
The analysis performed in this thesis work included the development of a biochemical and molecular screening test for laccase activity and a comparison with the lignocellulolytic basidiomycete Coprinus cinereus in biochemical and molecular assays.
The main issues to be assessed were:
• T. virens production of extracellular laccases in the presence of lignocellulosic biomass;
• the differential expression of the T. virens six putative laccase genes;
• the T. virens laccase production compared to the one of the model organism C. cinereus.
In order to accomplish these tasks, several experimental trials have been set during a hosting period at the laboratories of the Section for Sustainable Biotechnology - Copenhagen Institute of Technology, Aalborg University, Denmark. The general experimental design can be resumed as follows:
• growth of fungal cultures on both a lignocellulosic and non-lignocellulosic media;
• analysis of laccase activity according to the ABTS assay;
• RT-PCR with primers specific for the six T. virens putative laccase genes and C. cinereus lcc1 gene.
File