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Archivio digitale delle tesi discusse presso l’Università di Pisa

Tesi etd-08022020-120819


Tipo di tesi
Tesi di laurea magistrale LM5
Autore
MAROTTA, CARLO
URN
etd-08022020-120819
Titolo
Modulazione dell'espressione di microRNA da parte dell'oleaceina in cellule di melanoma cutaneo
Dipartimento
FARMACIA
Corso di studi
CHIMICA E TECNOLOGIA FARMACEUTICHE
Relatori
relatore Prof.ssa Nieri, Paola
correlatore Dott.ssa Carpi, Sara
correlatore Dott.ssa Polini, Beatrice
Parole chiave
  • anticancer activity
  • attività antitumorale
  • chemio-prevenzione
  • chemoprevention
  • cutaneous melanoma
  • espressione genica
  • extra virgin olive oil
  • gene expression
  • melanoma cutaneo
  • miRNA
  • oleacein
  • oleaceina
  • olio extravergine di oliva
  • polifenolo
  • polyphenol
Data inizio appello
30/09/2020
Consultabilità
Non consultabile
Data di rilascio
30/09/2090
Riassunto
Melanoma is an increasing health risk on a global scale which requires urgent attention. In particular, it has been found that melanoma incidence is increasing, especially among young man and women. Consequently, novel preventive/treatment modalities for this disease are needed. Several scientific evidences outlined that the levels of many microRNAs (miRNAs) are dysregulated in melanoma. In this context, the ability of polyphenols to regulate the levels of miRNAs in cancer could lead to the development of new preventive strategies/therapies. Mediterranean diet has already demonstrated to prevent many cardiometabolic diseases through the polyphenols contained in extra-virgin olive oil (EVOO). One of the most abundant secoiridoid present in EVOO, Oleacein (OA), has demonstrated anticancer activity against several tumors. This thesis aims at determining OA ability to modulate miRNAs levels in melanoma. OA increased the levels of miR-193a-3p, miR-193a-5p, miR-34a-5p, miR-16-5p and miR-155-5p, while decreased miR-214-3p levels in melanoma cell line 501-Mel. These modulatory effects may explain the inhibition of melanoma cell growth observed after treatment with OA in a previous study. In fact, it has been proven that through the regulation of this miRNAs, OA is able to influence the expression of many genes involved in apoptosis. Altogether these results provide further insights into the mechanism of action with which OA may contribute to the nutraceutical properties of EVOO and suggest that this secoiridoid may represent a lead molecule for design of new anti-melanoma compounds.
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