ETD system

Electronic theses and dissertations repository

 

Tesi etd-07252012-221451


Thesis type
Tesi di laurea magistrale
Author
PETRILLO, CAROLINA
URN
etd-07252012-221451
Title
Direct lineage reprogramming: from fibroblasts to cardiomyocytes
Struttura
BIOLOGIA
Corso di studi
BIOLOGIA MOLECOLARE E CELLULARE
Supervisors
correlatore Prof. Andreazzoli, Massimiliano
relatore Prof.ssa Batistoni, Renata
correlatore Prof.ssa Marracci, Silvia
Parole chiave
  • induced cardiomyocytes
  • reprogramming
  • lentivirus
  • molecular analysis
Data inizio appello
13/09/2012;
Consultabilità
Parziale
Data di rilascio
13/09/2052
Riassunto analitico
Isolation of fibroblasts from biopsies of WT and Golden Retriever Muscular Dystrophy (GRMD) dogs, the closest animal model to human Duchenne Muscular Dystrophy. Lentiviral production using 293 cell to produce vectors containing transcription factor genes involved in heart development, such as GATA4, MEF2C, TBX5 and GFP gene, as cell tracer. Cell infection by lentiviral technology and cellular selection. Molecular analysis by qRT-PCR, FACS, WB and immunofluorescence for cells and tissue sections of hearts injected with iCMs.
File