ETD

Archivio digitale delle tesi discusse presso l'Università di Pisa

Tesi etd-06252019-130040


Tipo di tesi
Tesi di laurea magistrale
Autore
MASCELLANI, ANNA
URN
etd-06252019-130040
Titolo
Differential expression gene analysis on sugar pathway in ripe Ficus carica L. cv Dottato fruits under salinity stress
Dipartimento
SCIENZE AGRARIE, ALIMENTARI E AGRO-AMBIENTALI
Corso di studi
BIOTECNOLOGIE VEGETALI E MICROBICHE
Relatori
relatore Dott. Bernardi, Rodolfo
relatore Dott.ssa Mascagni, Flavia
correlatore Dott.ssa Pistelli, Laura
Parole chiave
  • salt stress
  • sugar biosynthesis
  • Ficus carica
  • gene expression
Data inizio appello
15/07/2019
Consultabilità
Non consultabile
Data di rilascio
15/07/2089
Riassunto
Fig fruit (Ficus carica L., Moraceae) is commonly consumed in its fresh or dried form. The taste of it is largely influenced by various compounds within it, including organic acids and sugars. To date, there are no data evaluating sugar metabolism genes in mature fruits of plants cultivated under salinity stress. The data available is related to the analysis of the enzymatic activities of the most important enzymes and the amounts of sugars present in the fruit, whether ripening or mature.
In fruits, sorbitol and sucrose are the two main forms of photosynthetic and translocated carbon, and they may have different functions depending on the utilisation organ and its developmental stage. The role and interaction of sorbitol and sucrose metabolism was studied on ripe fruits of Ficus carica L. cv. Dottato exposed to 100 mM sodium chloride for 48 days. In this study, the variation in expression of different genes involved in the metabolism of sugars was investigated by means of relative Real-Time PCR. The main genes involved in the conversion of sucrose and sorbitol into fructose, glucose and UDP-glucose, and later to glucose-6-phosphate and fructose-1,6-biphosphate were analysed along with sucrose, sorbitol, mannitol and hexose transporters.
The analysis showed an increasing expression level of the metabolism of sorbitol and sucrose to glucose, UDP-glucose and fructose. The expression level of hexokinase was unchanged and phosphofructokinase was up-regulated. The expression levels of sucrose transporters and sorbitol transporters were increased, and hexose and mannitol transporters were stable. It is possible that sugars enter the fruit cells, and in those cells, they are metabolised to simple sugars. However, this can only be determined following sugar concentration analysis; a whole series of post-transcriptional adjustments may be involved.
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