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Tesi etd-06232020-113025


Thesis type
Tesi di specializzazione (3 anni)
Author
CARDINI, ELENA
URN
etd-06232020-113025
Title
Studio sierologico per la ricerca delle lentivirosi dei piccoli ruminanti nel Capriolo
Struttura
SCIENZE VETERINARIE
Corso di studi
SANITA' ANIMALE, ALLEVAMENTO E PRODUZIONI ZOOTECNICHE
Supervisors
relatore Prof. Mazzei, Maurizio
Parole chiave
  • caprioli
  • lentivirosi dei piccoli ruminanti
  • SRLV
  • ELISA
  • Sierologia
Data inizio appello
15/07/2020;
Consultabilità
Secretata d'ufficio
Data di rilascio
15/07/2090
Riassunto analitico
Small ruminant lentiviruses (SRLV) are widespread among domesticated sheep and goats worldwide. Infection of wild ruminants has been proposed as an actor in SRLV epidemiology, but studies are limited. The aim of this study was to investigate SRLV circulation in roe deer. Serum from blood samples originating from 20 free-living roe deer, collected in the provinces of Florence, Grosseto and Pisa, were serologically tested using a commercial indirect ELISA kit for SRLV in sheep and goat, been able to identify the circulating viral genotype (A, B, E). The ELISA method is a simple and effective test for virus diagnosis, but there are no kits validated in roe deer, nor in wild ruminants. In order to improve the sensibility of the kit, we modified the manufacturer’s protocol using peroxidase-conjugated protein G as conjugate antibody. The results show 3/20 positive samples, providing the first data in Italy on potential susceptibility of roe deer to SRLV, using a modified-commercial kit validates in domestic small ruminants. Further studies are needed to know whether adaptation of SRLV to free-living hosts may be associated with the acquisition of new viral strains and properties and to provide new tools to control SRLV spread in sheep and goat.
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