ETD system

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Tesi etd-06222015-134620


Thesis type
Tesi di laurea magistrale
Author
CACOPARDO, LUDOVICA
URN
etd-06222015-134620
Title
Design, Prototyping, Integration and Testing of a TEER Measurement System in a Bioreactor for Application to a Model of Retinal Stress
Struttura
INGEGNERIA DELL'INFORMAZIONE
Corso di studi
INGEGNERIA BIOMEDICA
Commissione
relatore Prof.ssa Pêgo, Ana Paula
correlatore Ing. Giusti, Serena
relatore Prof.ssa Ahluwalia, Arti Devi
Parole chiave
  • Bioreactors
  • TEER
  • Physiological Barriers
  • In-vitro models
Data inizio appello
17/07/2015;
Consultabilità
parziale
Data di rilascio
17/07/2018
Riassunto analitico
Physiological barrier have a fundamental role in human homeostasis, as they are the main gate for the passage of chemical compounds, as well as the first defence against toxic agents. For this reason, predictive in-vitro models of these barriers are very important to study drug and toxicants absorption, monitoring the integrity of the cell layer as an index for toxic or healthy effect. An established parameter to quantify the status of cell monolayer is the Trans Epithelial Electric Resistance (TEER), which evaluates the formation of tight junctions in endothelial and epithelial barriers. Classical in-vitro models are based on cells cultured on permeable supports in static conditions and TEER measurements are performed with a chopstick electrode. However, since dynamic models are nowadays affirming as the better way to mimic physiological conditions, the integration of TEER measurement systems in these devices is the new challenge. <br>In this work, silver electrode and a measuring system have been integrated in a bioreactor to perform standalone TEER measurement and monitor the monolayer integrity during time. The system was tested with Human Umbilical Vein Endothelial Cells (HUVECs) and Brain endothelial cells (bEnd3), evaluating TEER and cell viability in dynamic conditions for up to 5 days.<br>
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