ETD

Archivio digitale delle tesi discusse presso l'Università di Pisa

Tesi etd-04242009-150017


Tipo di tesi
Tesi di dottorato di ricerca
Autore
PACCHINI, SARA
URN
etd-04242009-150017
Titolo
Determination of non-enzymatic antioxidant molecules in animal semen and their correlations with seminal parameters and fertility
Settore scientifico disciplinare
VET/02
Corso di studi
MEDICINA VETERINARIA
Relatori
Relatore Prof. Ducci, Michele
Parole chiave
  • l-Histidine
  • kinetic parameters
  • Carnosine
  • animal semen
  • Taurine
Data inizio appello
12/06/2009
Consultabilità
Non consultabile
Data di rilascio
12/06/2049
Riassunto
In this Doctoral Thesis the natural presence of Carnosine and L-Histidine in seminal plasma and spermatozoa of boar, donkey and stallion was proved.
These molecules showed a positive effect, in nature, on some seminal parameters.
The artificial addition to BTS extender in boar fresh semen of these molecules demonstrated that Carnosine affect positively the kinetic parameters and the same happens with Taurine, instead l-Histidine demonstrated a negative effects.
Their additions to thawing extender in frozen-thawed boar spermatozoa showed that Taurine increased the sperm functionality, instead Carnosine could induce pro-oxidative process in high concentration with consequent negative effect on spermatozoa although no significant differences were showed in motility, viability or chromatin condensation.
In conclusion, Carnosine and Taurine appears to play an important role in the sperm antioxidant defence strategy.
Indeed addition of Carnosine to the extender in boar fresh semen and of Taurine to thawing extender in frozen boar semen could be of significant benefit in improving the function and fertilizing ability of boar spermatozoa.
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