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Tesi etd-04162010-143127


Tipo di tesi
Tesi di dottorato di ricerca
Autore
SANCHEZ, ANGEL MATIAS
URN
etd-04162010-143127
Titolo
RAPID SIGNALING OF ESTROGEN TO WAVE1 AND MOESIN CONTROLS NEURONAL SPINE FORMATION VIA THE ACTIN CYTOSKELETON
Settore scientifico disciplinare
MED/40
Corso di studi
FISIOPATOLOGIA DELLA RIPRODUZIONE E SESSUOLOGIA
Relatori
tutor Dott. Simoncini, Tommaso
controrelatore Prof. Acconcia, Filippo
controrelatore Prof.ssa Baldi, Elisabetta
relatore Prof. Genazzani, Andrea R.
Parole chiave
  • actin cytoskeleton
  • dentritic spines
  • Estrogen Receptor
  • moesin
  • WAVE-1
Data inizio appello
17/05/2010
Consultabilità
Completa
Riassunto
Estrogens are important regulators of neuronal cell morphology, and this is thought to be critical for gender-specific differences in brain function and dysfunction. Dendritic spine formation is dependent on actin remodeling by the WASP-family verprolin homologous (WAVE1) protein, which controls actin polymerization through the actin-related protein (Arp)-2/3 complex. Emerging evidence indicates that estrogens are effective regulators of the actin cytoskeleton in various cell types via rapid, extranuclear signaling mechanisms. We here show that 17-estradiol (E2) administration to rat cortical neurons leads to phosphorylation of WAVE1 on the serine residues 310, 397, and 441 and to WAVE1 redistribution toward the cell membrane at sites of dendritic spine formation. WAVE1 phosphorylation is found to be triggered by a Gαi/Gβ protein-dependent, rapid extranuclear signaling of estrogen receptor α to c-Src and to the small GTPase Rac1. Rac1 recruits the cyclin-dependent kinase (Cdk5) that directly phosphorylates WAVE1 on the three serine residues. After WAVE1 phosphorylation by E2, the Arp-2/3 complex concentrates at sites of spine formation, where it triggers the local reorganization of actin fibers. In parallel, E2 recruits a Gα13-dependent pathway to RhoA and ROCK-2, leading to activation of actin remodeling via the actin-binding protein, moesin. Silencing of WAVE1 or of moesin abrogates the increase in dendritic spines induced by E2 in cortical neurons. In conclusion, our findings indicate that the control of actin polymerization and branching via moesin or WAVE1 is a key function of estrogen receptor α in neurons, which may be particularly relevant for the regulation of dendritic spines.
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