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Digital archive of theses discussed at the University of Pisa

 

Thesis etd-03062009-122155


Thesis type
Tesi di dottorato di ricerca
Author
D'HUBERT, FRANCOIS
URN
etd-03062009-122155
Thesis title
Silencing of the cytosolic 5'nucleotidase II (cN-II)mediated by a shRNA-expressing lentiviral vector.
Academic discipline
BIO/11
Course of study
BIOTECNOLOGIE MOLECOLARI
Supervisors
Relatore Prof.ssa Tozzi, Maria Grazia
Keywords
  • cancer
  • cytosolic 5'nucleotidase
  • lentiviral vector
  • RNAi
Graduation session start date
26/02/2009
Availability
Withheld
Release date
26/02/2049
Summary
Cytosolic 5’-nucleotidase/phosphotransferase (cN-II), acting through the formation of a phospho-enzyme intermediate, is specific for oxy- and deoxypurine monophosphates. cN-II is an enzyme of great importance: overexpression of cN-II has negative effects on human cell survival. cN-II is involved in the regulation of intracellular concentration of IMP, AMP, GMP and also PRPP, therefore in the regulation of purine de novo synthesis and salvage. cN-II is clinically interesting, since it is involved in prodrug cellular metabolism. Besides, mRNA level of cN-II has a prognostic value in adult AML (acute myeloid leukemia). From this view point, the study of cN-II depletion constitutes a crucial step in the understanding of its functions in cell metabolism. The knock-down of cN-II revealed that its absence is lethal for cells, and that the addition of the product normally generated by the enzyme allows knocked-down cells to survive.
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