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Electronic theses and dissertations repository

 

Tesi etd-03062008-165141


Thesis type
Tesi di dottorato di ricerca
Author
PETRUCCI, ARIANNA
email address
a.petrucci@kedrion.com
URN
etd-03062008-165141
Title
Development of plasma protein isolation
Settore scientifico disciplinare
CHIM/08
Corso di studi
BIOMATERIALI
Commissione
Relatore Prof. Solaro, Roberto
Relatore Dott. Morelli, Andrea
Parole chiave
  • immunoglobulins
  • human plasma
  • chromatography
Data inizio appello
04/04/2008;
Consultabilità
parziale
Data di rilascio
04/04/2048
Riassunto analitico
The aim of this research project is to develop a new chromatography-based manufacturing process of an immunoglobulin G (IgG) concentrate for intravenous administration, starting from human plasma. The presented experimental data shows that it is possible to purify IgG from an upstream plasma fraction (obtained by Cohn’s fractionation) by two chromatographic steps (a capture step and a polishing step), and to formulate it properly for its intended use. The general approach to protein purification, the international guidelines about manufacturing and viral safety of biopharmaceutical products, and the most recent marketing suggestions were taken as a reference during the development work.
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