ETD system

Electronic theses and dissertations repository

 

Tesi etd-03032012-102431


Thesis type
Tesi di dottorato di ricerca
Author
SBRANA, TOMMASO
URN
etd-03032012-102431
Title
Fluidic systems design for physiologically relevant in-vitro models
Settore scientifico disciplinare
ING-IND/34
Corso di studi
AUTOMATICA, ROBOTICA E BIOINGEGNERIA
Commissione
tutor Prof.ssa Ahluwalia, Arti Devi
Parole chiave
  • multiwell
  • in-vitro model
  • gastro-intestinal tract
  • fluid dynamic simulation
  • dynamic cell culture
  • bioreactor
  • nanoparticle deposition
Data inizio appello
12/04/2012;
Consultabilità
completa
Riassunto analitico
This PhD thesis is focused on the design, realization and characterization of fluidic devices that could be useful to develop new physiologically relevant in-vitro models. The word “relevant” in the title is referred to the correspondence between in-vitro models and physiology. In fact in a multi organ models it is important to maintain physiological ratios between tissues. These ratios can be correlated to cell numbers, to cell seeding surface areas or to the volume of fresh blood that reaches each tissue. Allometry is the science that permits the establishment of ratios between physiological vales and in-vitro characteristics. In my thesis some in-vitro models are studied using an allometric approach in order to analyze cross talk between different cell types.<br><br>During my thesis several new bioreactors were studied and improved in order to establish new in-vitro models for the physiological barriers and cross talk between different organs. The last part of the thesis is dedicated to the design and realization of a new system. To facilitate biological and cell culture studies, this new system attempts to merge bioreactors technical evolutions with common cell culture multiwell plate specifications and standard protocol of analysis. The innovative device has been patented.<br>
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