logo SBA

ETD

Archivio digitale delle tesi discusse presso l’Università di Pisa

Tesi etd-02042024-152720


Tipo di tesi
Tesi di laurea magistrale
Autore
DI LORENZO, GIULIA
URN
etd-02042024-152720
Titolo
Preparation of wood distillate (pyroligneous acid) microcapsules coated with chitosan and biochar and evaluation of their effects against the soil-borne fungal pathogen Macrophomina phaseolina
Dipartimento
SCIENZE AGRARIE, ALIMENTARI E AGRO-AMBIENTALI
Corso di studi
BIOTECNOLOGIE VEGETALI E MICROBICHE
Relatori
relatore Dott.ssa Pecchia, Susanna
relatore Dott. Becagli, Michelangelo
correlatore Prof. Cardelli, Roberto
Parole chiave
  • antifungal activity
  • chitosan
  • microcapsules
  • micronized biochar
  • wood distillate
Data inizio appello
19/02/2024
Consultabilità
Non consultabile
Data di rilascio
19/02/2027
Riassunto
Wood distillate (WD), is a liquid byproduct of charcoal production. It has antimicrobial and antifungal properties thanks to its combination of over 300 components, including organic acids, polyphenols, and tannins. Chitosan (CH) is a biopolymer with antioxidant properties that can improve plant defence mechanisms, while biochar (BC) is a carbon-rich material that can improve the bioavailability of soil nutrients and crop productivity. The aim of this work was to develop WD-containing microcapsules coated with CH or CH+micronized BC, and finally test their effects against the soil-borne fungal pathogen Macrophomina phaseolina.
Microencapsulation of WD was performed by ion exchange gelation method, using sodium alginate as the polymer. The microcapsules were coated with a CH solution or a CH + micronized BC suspension. Three types of microcapsules were produced: uncoated, CH-coated, or CH-BC-coated WD microcapsules. In vitro experiments were conducted to observe their solubilization through pH measurements, while their effects were evaluated on the in vitro growth of M. phaseolina.
The microencapsulation of the WD and the formation of the microcapsule coating were successfully performed. WD microcapsules coated with CH and micronized BC, provided a significant reduction in the in vitro growth of M. phaseolina. Further investigations on the effects of these microcapsules against soil-borne pathogens could be conducted in light of their potential use in sustainable agriculture.
File