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Tesi etd-04212010-113204


Thesis type
Tesi di dottorato di ricerca
Author
ROGNONI, SARA
URN
etd-04212010-113204
Title
Analysis of Sugar Sensing Using Arabidopsis thaliana natural variation
Settore scientifico disciplinare
BIO/04
Corso di studi
BIOTECNOLOGIE MOLECOLARI
Commissione
tutor Prof. Perata, Pierdomenico
Parole chiave
  • Nessuna parola chiave trovata
Data inizio appello
16/03/2010;
Consultabilità
parziale
Data di rilascio
16/03/2050
Riassunto analitico
Although it has already been shown that sugars have not only a metabolic function in plants,<br>little is known about the mechanisms by which plants respond to them. High concentrations<br>of sugars arrest the development of young Arabidopsis thaliana seedlings. In order to better<br>understand this process, we exploited the natural variation of two Arabidopsis accessions<br>differing largely in their response to sugars: the laboratory strain Landsberg erecta (Ler),<br>less sensitive, and the more sensitive accession Cape Verde Islands (Cvi). With a set of<br>recombinant inbred lines (RILs) derived from a cross between both accessions, quantitative<br>trait loci (QTLs) for the sugar induced developmental arrest have been identified and then<br>confirmed analysing near isogenic lines (NILs). The QTL GSQ5 co-locates with the QTL for<br>seed dormancy DOG1. There is a positive feedback loop between sugar-ABA signal<br>cascade and Cvi allele of GSQ5/DOG1. Sugar induced the expression of GSQ5/DOG1-Cvi<br>via sugar-ABA signalling cascade, and GSQ5/DOG1-Cvi enhances the sugar-ABA signalling<br>cascade through the induction of ABI4. At the same time, fine mapping of another QTL<br>specific for fructose is currently in progress.
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