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Tesi etd-03172014-204337


Thesis type
Tesi di laurea magistrale
Author
RELLA, GIULIA
URN
etd-03172014-204337
Title
Fabrication and characterization of fibrous poly(ε-caprolactone)/poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) scaffold for cardiac patch application
Struttura
CHIMICA E CHIMICA INDUSTRIALE
Corso di studi
CHIMICA INDUSTRIALE
Supervisors
relatore Prof.ssa Chiellini, Federica
controrelatore Prof. Solaro, Roberto
correlatore Prof. Boccaccini, Aldo
Parole chiave
  • poly(ε-caprolactone)
  • electrospinning
  • scaffolds
  • biomaterials
  • poly(3-hydroxybutyrate-co-3-hydroxyhexanoate)
  • polyhydroxyalkanoates
  • cardiac tissue engineering
Data inizio appello
10/04/2014;
Consultabilità
Completa
Riassunto analitico
The main goal of this thesis was the development and characterization of highly porous scaffolds for cardiac tissue regeneration using a blend of two different biopolymers. The investigated blend was based on the chemically synthesized polymer poly(ε-caprolactone) (PCL) and the natural polymer poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx). PCL is an important and extensively investigated polymer for tissue engineering applications while PHBHHx has demonstrated good biocompatibility and attractive mechanical properties. Electrospinning technique was chosen to develop biodegradable cardiac patches with high porosity. The electrospinning conditions such as polymer concentration, applied voltage and distance from the collector were investigated and optimized and subsequently the fibrous scaffolds were characterized. The characterization of the materials involved microstructural analysis and investigation of the material degradation behavior in relevant fluids. Furthermore the mechanical properties of the developed patches were investigated, e.g. stiffness and stress-strain displacement curves and fracture characteristics. Finally cytocompatibility of the blend was assessed in vitro by studying adhesion and proliferative capacity of C2C12 cells on the surface of the fibrous cardiac scaffolds.
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