Tesi etd-11222013-135758 |
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Tipo di tesi
Tesi di laurea magistrale
Autore
PELLEGRINI, DAVIDE
URN
etd-11222013-135758
Titolo
Development of a chromatographic method for the determination of lactate in urine and saliva
Dipartimento
CHIMICA E CHIMICA INDUSTRIALE
Corso di studi
CHIMICA
Relatori
relatore Prof.ssa Colombini, Maria Perla
relatore Dott.ssa Bramanti, Emilia
controrelatore Prof. Fuoco, Roger
relatore Dott.ssa Bramanti, Emilia
controrelatore Prof. Fuoco, Roger
Parole chiave
- 9-CMA
- creatinine
- DAD
- exercise
- experimental design
- FD
- HPLC-ESI-q-ToF
- intensive care
- lactate
- RP-HPLC
- saliva
- urine
Data inizio appello
14/12/2013
Consultabilità
Completa
Riassunto
The aim of this study was to develop a novel method for the determination of lactic acid in biological matrices as urine and saliva using a pre-column derivatization procedure with 9-chloro-methyl-anthracene (9-CMA) and liquid chromatography (RP-HPLC) with UV detection at 365 nm and fluorescence detection at 410 nm (λex = 365 nm).
The formation of the lactate derivative and by products was confirmed by LC-ESI-q-ToF.
The formation of the lactate derivative and by products was confirmed by LC-ESI-q-ToF.
File
| Nome file | Dimensione |
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| TESI_FIN...RTINA.pdf | 2.82 Mb |
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