ETD

Archivio digitale delle tesi discusse presso l'Università di Pisa

Tesi etd-10012019-130948


Tipo di tesi
Tesi di laurea magistrale
Autore
ZERINO, CATERINA
URN
etd-10012019-130948
Titolo
Pt(II) complexes as potential precursors of anticancer agents and their interaction with model proteins
Dipartimento
CHIMICA E CHIMICA INDUSTRIALE
Corso di studi
CHIMICA
Relatori
relatore Prof.ssa Tinè, Maria Rosaria
correlatore Prof.ssa Gabbiani, Chiara
controrelatore Prof.ssa Biver, Tarita
Parole chiave
  • Pt(II)
  • mass spectrometry
  • DSC
  • calorimetry
  • anticancer agents
  • UV-Vis titrations
Data inizio appello
22/10/2019
Consultabilità
Completa
Riassunto
Over the past 30 years, platinum-based drugs, particularly cisplatin and carboplatin, have been widely used in chemotherapy to treat various cancers. The use of cisplatin is limited by the presence of several side effects and by the development of long-term cisplatin resistance. For this reason, the research is looking for new platinum-based anticancer drugs to improve the clinical performance of cisplatin. In addition, the interaction of anticancer platinum complexes with proteins is of great importance, since the proteins have multiple functions in the activity of cisplatin and they are involved in many stages of the cytotoxic process. In this project three metal complexes based on Pt(II) were synthesized and their interaction with two model proteins (lysozyme and ribonuclease A) were studied, using differential scanning calorimetry, UV-Vis absorption titrations and mass spectrometry. Based on the results obtained, the compound 1 and the compound 2 interact strongly with the model proteins by coordination with cysteine or histidine residues, while the compound 3 interacts only weakly with the model proteins. Furthermore, binding constants and thermodynamic parameters were calculated by differential scanning calorimetry. The calorimetric binding constants were compared with those obtained by spectroscopic studies.
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