ETD

Archivio digitale delle tesi discusse presso l'Università di Pisa

Tesi etd-03252015-132010


Tipo di tesi
Tesi di laurea magistrale
Autore
GIUSTI, MICHELA
URN
etd-03252015-132010
Titolo
Determination of lactate in urine and saliva by derivatization with 9-chloromethyl anthracene (9-CMA) and liquid chromatography with fluorescence detection. New apllications in clinical chemistry and sport.
Dipartimento
CHIMICA E CHIMICA INDUSTRIALE
Corso di studi
CHIMICA
Relatori
relatore Bramanti, Emilia
controrelatore Petri, Antonella
relatore Prof.ssa Giannarelli, Stefania
Parole chiave
  • determination
  • urine
  • saliva
  • lactate
  • 9-chloromethyl anthracene
Data inizio appello
14/04/2015
Consultabilità
Completa
Riassunto
The present study, reports the application of a method for the determination of lactate in biological matrices (urine and saliva) using a pre-column derivatization procedure with 9-chloromethyl anthracene (9-CMA) and liquid chromatography (RP-HPLC) with fluorescence detection at 410 nm (λex = 365 nm).
The calibration curve was linear in the investigated range 0.05-5.5 mM and the limit of detection was 0.04 mM (slope = 5.19±0.08•105, R2 = 1.000). Intra-day and inter-day repeatability were less than 4 and 3 %, respectively. The calibration curves in urine samples and saliva samples were linear in the same range 0.05-5.5 mM (slope = 3.30±0.17•105, R2 = 0.994 for urine; slope = 5.29±0.26•105, R2 = 0.998 for saliva). Intra-day repeatability was less than 5% (urine) and 3% (saliva). Intra-day recoveries were found to be between 82 and 100 % (urine) and 89 and 102 % (saliva).
The optimized method for the lactate determination was successfully applied to urine of patients from an intensive care unit of Cisanello Hospital (Pisa), in order to determine the correlation between the concentration of lactate in the blood and in urine, and to saliva sampled in several athletes during intensive training.
File